Automating Workflows with Sequence Files in Chromperfect

One of the most powerful features of Chromperfect is the ability to create and use sequence files. A sequence file is essentially a structured list of injections that automates sample acquisition and processing. By using them, laboratories can streamline workflows, reduce manual entry, and ensure consistency across analytical runs.

Why Use Chromperfect Sequence Files?

A sequence file is more than just a list of samples. It:

• Automates the acquisition and processing of multiple injections.

• Updates calibration files before analyzing unknown samples.

• Ensures replicates, standards, and levels are applied consistently.

• Improves efficiency and reduces the chance of human error.

Calibration and Sample Lists

A typical sequence begins with calibration injections. For example:

• Two calibration standards, each injected twice, produce four calibration runs.

• These runs update the calibration file at different levels.

After calibration, sample injections are added. These use the updated calibration file for analysis but are not used to modify the calibration itself.

If an instrument has multiple channels, the calibration lists must remain consistent across all channels. Chromperfect automatically expands the table to reflect this when multiple channels are specified.

Building a Sequence File Step by Step

1. Open the File Editor → Create a new sequence file.

2. Choose “Empty File” → A spreadsheet view appears.

3. Insert Entries → Add rows for each injection (calibration + samples).

4. Adjust Channels → Set the number of channels; rows expand accordingly.

5. Enter Core Parameters (required for each line):

   • Raw file name

   • Cycle number

   • Method file name

   • Calibration level

   • (Optional but useful: sample name; required if using an autosampler: vial number)

6. Use Copy Down Tools → Speed up entry with “copy down” and “copy down with increment.”

7. Save the File → For example, MyTest.seq.

8. Run the Sequence → Click “Run” to begin automated acquisition and processing.

Practical Benefits in the Lab

By preparing a sequence file in advance, laboratories can:

• Run multiple calibration standards and samples unattended.

• Ensure traceable, consistent updates to calibration.

• Improve reproducibility and save analyst time.

Sequence files are fundamental for scaling up laboratory operations and making the most of Chromperfect’s automation features.