Chromatography Theory and Training

Introduction to chromatography peak theory: learn definitions of area, height, width, skew, tailing factor, kurtosis, resolution, and theoretical plates.

How digitization choices shape chromatography data: get sampling rate, range, resolution, and dynamic range right to preserve peak height, area, and retention time.

How detector noise biases baselines—and how to fix it with the right smoothing algorithm and window size in Chromperfect. Includes noise/RMS measurement, S/N, Savitzky–Golay derivatives, and when smoothing should (and shouldn’t) be used.

How Chromperfect’s timed events refine peak detection and baselines in tough chromatograms—covering CLAMP/FLIP, PW/TH/MUT, INT/HORZ/NEG, SBN/SBNV, SBAV/COM, and SKIM—plus when to use (and avoid) each.

Chromatography Theory. Peak Integration Overview This chapter closes out Integration Theory with two critical steps: Peak Identification...

Master chromatography calibration in Chromperfect: linear vs. non-linear fits, weighting for low levels, origin handling, precision metrics, troubleshooting, and internal standard strategies.