If a chromatography peak suddenly develops a flat top, detector overload is an obvious suspect — but it is not the only possible cause. A flat top chromatography peak can originate in the detector itself, in the analog data acquisition hardware, or, in one specific historical case, within the chromatography data system. Correctly identifying where the signal is being limited matters because each cause requires a different solution. Diluting a sample will not correct an acqui

Learn how SEC integration theory converts chromatographic detector response into molecular-weight information, including SEC baselines, noise treatment, internal-standard flow correction, detector-response types, molecular-weight averages and molecular-weight fractions.

Learn how calibration works in Chromperfect and how it links detector response to concentration for accurate and repeatable quantification.